β-Tubulin mRNA as a Marker of Cryptosporidium parvum Oocyst Viability
نویسندگان
چکیده
منابع مشابه
Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability.
Purified Cryptosporidium parvum oocysts were exposed to ozone, chlorine dioxide, chlorine, and monochloramine. Excystation and mouse infectivity were comparatively evaluated to assess oocyst viability. Ozone and chlorine dioxide more effectively inactivated oocysts than chlorine and monochloramine did. Greater than 90% inactivation as measured by infectivity was achieved by treating oocysts wit...
متن کاملIsolation of Cryptosporidium parvum Oocyst From Infected Feces
Introduction Cryptosporidium is a genus of Apicomplexa phylum parasites that can cause a respiratory and gastrointestinal illness (cryptosporidiosis) that primarily involves watery diarrhea (intestinal cryptosporidiosis) with or without a persistent cough (respiratory cryptosporidiosis) in both immunocompetent and immunodeficient humans (1-3). A number of Cryptosporidium species infect mammals ...
متن کاملIsolation of Small Number of Cryptosporidium parvum Oocyst Using Immunochromatography
BACKGROUND Cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. The organism can be transmitted through water. Since small number of C. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of C. parvum oocyst in samples with limited number of oocysts. METHODS Antibody was prepared against ...
متن کاملisolation of small number of cryptosporidium parvum oocyst using immunochromatography.
background: cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. the organism can be transmitted through water. since small number of c. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of c. parvum oocyst in samples with limited number of oocysts. methods: antibody was prepared against ...
متن کاملEffects of low temperatures on viability of Cryptosporidium parvum oocysts.
Microcentrifuge tubes containing 8 x 10(6) purified oocysts of Cryptosporidium parvum suspended in 400 microliters of deionized water were stored at 5 degrees C for 168 h or frozen at -10, -15, -20, and -70 degrees C for 1 h to 168 h and then thawed at room temperature (21 degrees C). Fifty microliters containing 10(6) oocysts was administered to each of five to seven neonatal BALB/c mice by ga...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1999
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.65.4.1584-1588.1999